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jove.com
Video: DNA Ligation Reactions: Principle, Procedure, and Applications
197.3K Views. In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular ...
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Feb 1, 2013
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Sep 16, 2024
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